Fe3O4 Magnetic Dextran Nanoparticles Modified with SPA Ligand for IgG Purification

Abstract

Abstract: The objective of present research is to prepare magnetic dextran nanoparticles conjugated with Staphylococcal Protein A（SPA-MDPs）for the purification of IgG. The magnetic nanoparticles were prepared by following a high-temperature solution-phase procedure, and the average particle size can be tuned from 30 to 200 nm by simply varying the concentration of NaOH during the reaction. The size of SPA-MDPs nanoparticles has a great influence on their adsorption capacity for IgG. A maximum adsorption of human IgG was calculated to be 84.85 mg/mL with a binding affinity constant of 3.48×10⁶ M^-1 when the average size of SPA-MDPs is about 101.5 nm. The experiment results also demonstrated that the SPA-MDPs nanoparticles can be recycled for 5 times with minor loss of adsorption capacity. Moreover, IgG was purified from human plasma with a purity of 93.5% and recovery of 88.7%. Thus, SPA-MDPs show promising potentials for isolation and purification of IgG.

Key words: Dextran, Magnetic nanoparticles, SPA, IgG purification

Ren Jinggang, Cheng Chao, Zhong Conghao, Zhang li, Li Rongxiu. Fe₃O₄ Magnetic Dextran Nanoparticles Modified with SPA Ligand for IgG Purification[J]. Biotechnology Bulletin, 2014, 0(7): 201-208.

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Fe₃O₄ Magnetic Dextran Nanoparticles Modified with SPA Ligand for IgG Purification

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