Effects of Total Glycosides of Qiwei Baizhu Powder on Intestinal Microbiota and Enzyme Activities in Diarrhea Mice

doi:10.13560/j.cnki.biotech.bull.1985.2021-0149

Abstract

Abstract:

To explore the material basis of Qiwei Baizhu Powder（QWBZP）in dysbacteria diarrhea treatment,the total glycosides（TG）from QWBZP was extracted,and the effects of TG on the intestinal microecology of diarrhea mice were investigated. The results showed that TG was conducive to restoring the food intake and body weight of diarrhea mice. TG in medium dose helped the spleen index restored to normal level. All doses of TG promoted the proliferation of Bifidobacterium,Lactobacillus and Escherichia coli,and TG in a low dose significantly promoted the proliferation of Bifidobacterium（P<0.01）and Lactobacillus（P<0.05）. All doses of TG increased intestinal microbial activities（P<0.01）,lactase activities（P<0.01）and sucrase activities（P<0.01 or P<0.05）in the mice with diarrhea. This study revealed that the effects of TG on beneficial bacteria,intestinal microbial activities and enzyme activities of the diarrhea mice were similar to that by the decoction of QWBZP. Glycosides are important pharmacological component of QWBZP in dysbacteria diarrhea treatment.

Key words: total glycosides of Qiwei Baizhu Powder, intestinal microbiota, intestinal microbial activity, enzyme activity

XIE Guo-zhen, TANG Yuan, WU Yi, HUANG Li-li, TAN Zhou-jin. Effects of Total Glycosides of Qiwei Baizhu Powder on Intestinal Microbiota and Enzyme Activities in Diarrhea Mice[J]. Biotechnology Bulletin, 2021, 37(12): 124-131.

Figures/Tables 7

References 22

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分组Group	初始体重Initial weight/g	增重率 Weight gain rate/%
分组Group	初始体重Initial weight/g	适应期Adaptive phase	造模期Modeling phase	治疗期Treatment phase
N	18.57 ± 0.95	35.19 ± 7.12	20.59 ± 7.55	6.80 ± 2.69
R	18.34 ± 0.64	34.66 ± 4.94	12.20 ± 4.12	14.55 ± 2.53 A
TW	18.20 ± 0.48	38.78 ± 2.84	14.35 ± 6.09	11.36 ± 8.66
TL	18.17 ± 0.41	34.95 ± 1.41	15.43 ± 8.54	9.92 ± 3.36
TM	17.81 ± 0.83	34.74 ± 3.49	15.11 ± 6.55	11.18 ± 9.78
TH	18.01 ± 0.74	37.18 ± 4.56	13.36 ± 7.34	9.70 ± 10.13

分组Group	初始体重Initial weight/g	增重率 Weight gain rate/%
分组Group	初始体重Initial weight/g	适应期Adaptive phase	造模期Modeling phase	治疗期Treatment phase
N	18.57 ± 0.95	35.19 ± 7.12	20.59 ± 7.55	6.80 ± 2.69
R	18.34 ± 0.64	34.66 ± 4.94	12.20 ± 4.12	14.55 ± 2.53 A
TW	18.20 ± 0.48	38.78 ± 2.84	14.35 ± 6.09	11.36 ± 8.66
TL	18.17 ± 0.41	34.95 ± 1.41	15.43 ± 8.54	9.92 ± 3.36
TM	17.81 ± 0.83	34.74 ± 3.49	15.11 ± 6.55	11.18 ± 9.78
TH	18.01 ± 0.74	37.18 ± 4.56	13.36 ± 7.34	9.70 ± 10.13

分组 Group	脾脏指数 Spleen index/%	胸腺指数 Thymus index/%	肝脏指数 Liver index/%
N	0.30 ± 0.06	0.38 ± 0.04	5.57 ± 0.29
R	0.38 ± 0.04	0.37 ± 0.08	5.30 ± 0.29
TW	0.31 ± 0.03 b	0.41 ± 0.03	5.21 ± 0.69
TL	0.39 ± 0.10	0.35 ± 0.07	5.16 ± 0.45
TM	0.33 ± 0.05	0.42 ± 0.05	5.08 ± 0.36
TH	0.41 ± 0.03 AC	0.41 ± 0.05	5.58 ± 0.34

分组 Group	脾脏指数 Spleen index/%	胸腺指数 Thymus index/%	肝脏指数 Liver index/%
N	0.30 ± 0.06	0.38 ± 0.04	5.57 ± 0.29
R	0.38 ± 0.04	0.37 ± 0.08	5.30 ± 0.29
TW	0.31 ± 0.03 b	0.41 ± 0.03	5.21 ± 0.69
TL	0.39 ± 0.10	0.35 ± 0.07	5.16 ± 0.45
TM	0.33 ± 0.05	0.42 ± 0.05	5.08 ± 0.36
TH	0.41 ± 0.03 AC	0.41 ± 0.05	5.58 ± 0.34

分组 Group	细菌 Bacteria /（10⁶CFU·g^-1）	大肠杆菌 Escherichia coli /（10⁶CFU·g^-1）	双歧杆菌 Bifidobacterium /（10⁷CFU·g^-1）	乳酸菌 Lactobacillus /（10⁷CFU·g^-1）
N	1.10 ± 0. 10	0.81 ± 0.07	2.79 ± 0.82	4.53 ± 1.14
R	2.63 ± 0.21 A	0.97 ± 0.06 a	0.57 ± 0.08 a	0.65 ± 0.09 a
TW	2.04 ± 0.15 Ab	0.84 ± 0.02 b	0.60 ± 0.05 a	1.22 ± 0.78 a
TL	0.34 ± 0.11 ABC	9.09 ± 0.30 ABC	2.40 ± 0.04 BC	8.20 ± 2.80 bc
TM	15.37 ± 0.50 ABCD	2.90 ± 0.07 ABCD	2.70 ± 1.99	5.37 ± 0.35 BC
TH	2.83 ± 1.20 dE	1.21 ± 0.07 AbCDE	2.80 ± 1.84	0.96 ± 0.07 aBdE