Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (12): 252-262.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0211
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LIU Shuang(), YAO Jia-ni, SHEN Cong, DAI Jin-xia()
Online:
2022-12-26
Published:
2022-12-29
Contact:
DAI Jin-xia
E-mail:1849468755@qq.com;daijx@163.com
LIU Shuang, YAO Jia-ni, SHEN Cong, DAI Jin-xia. Fluorescent Quantitative PCR of nifH Gene and Diversity Analysis of Nitrogen-fixing Bacteria in the Rhizosphere Soil of Caragana spp. of Desert Grassland[J]. Biotechnology Bulletin, 2022, 38(12): 252-262.
样地Sampling site | Ct值Ct value | 拷贝数Number of copies/(copies·μL-1 DNA) | 称重Weight/g | 拷贝数Number of copies/(copies·g-1 ) |
---|---|---|---|---|
LS | 22.76 | 3.7298E+03 | 0.27 | 1.5922E+06a |
HSP | 25.67 | 6.3276E+02 | 0.38 | 1.7188E+05b |
SPT | 25.36 | 7.4268E+02 | 0.48 | 1.5550E+05b |
ZQ | 25.41 | 7.1586E+02 | 0.28 | 2.6036E+05b |
TSH | 24.47 | 1.4555E+03 | 0.30 | 5.2318E+05b |
Table 1 Ct values and quantitative results of nifH gene
样地Sampling site | Ct值Ct value | 拷贝数Number of copies/(copies·μL-1 DNA) | 称重Weight/g | 拷贝数Number of copies/(copies·g-1 ) |
---|---|---|---|---|
LS | 22.76 | 3.7298E+03 | 0.27 | 1.5922E+06a |
HSP | 25.67 | 6.3276E+02 | 0.38 | 1.7188E+05b |
SPT | 25.36 | 7.4268E+02 | 0.48 | 1.5550E+05b |
ZQ | 25.41 | 7.1586E+02 | 0.28 | 2.6036E+05b |
TSH | 24.47 | 1.4555E+03 | 0.30 | 5.2318E+05b |
样地 Sampling site | 有效序列数No. of valid sequences | 操作分类单元数目 No. of OTU | 覆盖度Coverage | 香农指数 Shannon index | 辛普森指数 Simpson index | Ace 指数 Ace index | Sobs指数 Sobs index | Chao1指数 Chao1 index |
---|---|---|---|---|---|---|---|---|
LS | 32 736 | 546 | 0.98864 | 3.2507b | 0.076655a | 546.53b | 320.00b | 462.98b |
SPT | 56 020 | 2 320 | 0.98389 | 4.9285a | 0.031739b | 1 459.70a | 1 244.70a | 1 415.70a |
ZQ | 62 271 | 2 703 | 0.98551 | 4.6876a | 0.047986ab | 1 468.50a | 1 266.70a | 1 454.50a |
TSH | 52 969 | 1 546 | 0.98491 | 4.0688ab | 0.040206b | 981.44ab | 710.67ab | 942.75ab |
HSP | 60 767 | 2 462 | 0.98696 | 4.3394a | 0.049855ab | 1 281.00a | 1 077.70a | 1 249.90a |
Table 2 Number of OTUs and Alpha diversity index of nitrogen-fixing bacteria at different sample sites
样地 Sampling site | 有效序列数No. of valid sequences | 操作分类单元数目 No. of OTU | 覆盖度Coverage | 香农指数 Shannon index | 辛普森指数 Simpson index | Ace 指数 Ace index | Sobs指数 Sobs index | Chao1指数 Chao1 index |
---|---|---|---|---|---|---|---|---|
LS | 32 736 | 546 | 0.98864 | 3.2507b | 0.076655a | 546.53b | 320.00b | 462.98b |
SPT | 56 020 | 2 320 | 0.98389 | 4.9285a | 0.031739b | 1 459.70a | 1 244.70a | 1 415.70a |
ZQ | 62 271 | 2 703 | 0.98551 | 4.6876a | 0.047986ab | 1 468.50a | 1 266.70a | 1 454.50a |
TSH | 52 969 | 1 546 | 0.98491 | 4.0688ab | 0.040206b | 981.44ab | 710.67ab | 942.75ab |
HSP | 60 767 | 2 462 | 0.98696 | 4.3394a | 0.049855ab | 1 281.00a | 1 077.70a | 1 249.90a |
Fig. 2 Venn diagram(A)and column diagram(B)of community composition of nitrogen-fixing bacteria in the rhizosphere soil of Caragana spp. at family level
速效氮 Availliable N /(mg·kg-1) | 速效磷 Availliable P /(mg·kg-1) | 速效钾 Availliable K /(mg·kg-1) | 有机质 Soil organic Matter/(g·kg-1) | 总氮 Total N /(g·kg-1) | 总磷 Total P /(g·kg-1) | 总钾 Total K /(g·kg-1) | pH | |
---|---|---|---|---|---|---|---|---|
nifH | 0.102 | 0.942* | 0.832 | 0.965** | 0.977** | 0.225 | 0.388 | 0.455 |
Table 3 Pearson correlation analysis between nifH gene copy number and soil physicochemical properties
速效氮 Availliable N /(mg·kg-1) | 速效磷 Availliable P /(mg·kg-1) | 速效钾 Availliable K /(mg·kg-1) | 有机质 Soil organic Matter/(g·kg-1) | 总氮 Total N /(g·kg-1) | 总磷 Total P /(g·kg-1) | 总钾 Total K /(g·kg-1) | pH | |
---|---|---|---|---|---|---|---|---|
nifH | 0.102 | 0.942* | 0.832 | 0.965** | 0.977** | 0.225 | 0.388 | 0.455 |
Fig. 4 Heatmap chart of correlation analysis between nitr-ogen-fixing bacteria and environmental factors AN:Availliable N;AP:availliable P;AK:availliable K;SOM:soil organic matter;TN:total N;TP:total P;TK:total K
菌株编号 Strain No. | 革兰氏染色 Gram stain | H2O2酶实验 Catalase test | 淀粉水解 Starch hydrolysis | V.P实验 V.P test | 甲基红实验 Methyl red test | 吲哚实验 Indole test | 硝酸盐还原 Nitrate reduction |
---|---|---|---|---|---|---|---|
LG-H2 | - | + | - | - | - | - | - |
LG-H3 | + | - | - | - | - | - | - |
LG-H4 | + | + | - | - | + | + | + |
LG-H5 | + | + | - | - | + | + | + |
LG-L3 | - | + | - | + | - | - | - |
LG-T2 | - | + | - | - | - | - | + |
LG-T3 | - | + | - | - | - | - | + |
LG-T6 | - | + | - | - | + | - | + |
LG-Z1 | - | + | - | - | - | - | + |
LG-Z3 | - | + | - | - | - | - | - |
LG-Z5 | - | + | - | + | - | - | + |
LG-Z7 | + | + | + | - | - | - | + |
GY-T1 | - | + | - | - | - | - | + |
GY-T4 | - | + | - | - | - | - | + |
GY-T5 | + | + | - | - | + | - | + |
GY-L1 | - | + | - | - | - | - | + |
GY-L5 | + | + | - | + | - | - | + |
GY-H3 | - | + | - | + | - | - | - |
GY-H8 | + | + | - | - | + | + | - |
GY-Z2 | - | + | - | - | - | - | + |
WD-L1 | - | - | - | - | - | - | - |
WD-L2 | - | + | - | - | - | - | - |
WD-Z1 | - | - | - | - | - | - | - |
WD-T3 | - | + | - | - | - | + | - |
WD-H1 | - | - | - | - | - | - | - |
YMA-T2 | - | + | - | - | - | - | + |
Table 4 Physiological and biochemical characteristics of strains
菌株编号 Strain No. | 革兰氏染色 Gram stain | H2O2酶实验 Catalase test | 淀粉水解 Starch hydrolysis | V.P实验 V.P test | 甲基红实验 Methyl red test | 吲哚实验 Indole test | 硝酸盐还原 Nitrate reduction |
---|---|---|---|---|---|---|---|
LG-H2 | - | + | - | - | - | - | - |
LG-H3 | + | - | - | - | - | - | - |
LG-H4 | + | + | - | - | + | + | + |
LG-H5 | + | + | - | - | + | + | + |
LG-L3 | - | + | - | + | - | - | - |
LG-T2 | - | + | - | - | - | - | + |
LG-T3 | - | + | - | - | - | - | + |
LG-T6 | - | + | - | - | + | - | + |
LG-Z1 | - | + | - | - | - | - | + |
LG-Z3 | - | + | - | - | - | - | - |
LG-Z5 | - | + | - | + | - | - | + |
LG-Z7 | + | + | + | - | - | - | + |
GY-T1 | - | + | - | - | - | - | + |
GY-T4 | - | + | - | - | - | - | + |
GY-T5 | + | + | - | - | + | - | + |
GY-L1 | - | + | - | - | - | - | + |
GY-L5 | + | + | - | + | - | - | + |
GY-H3 | - | + | - | + | - | - | - |
GY-H8 | + | + | - | - | + | + | - |
GY-Z2 | - | + | - | - | - | - | + |
WD-L1 | - | - | - | - | - | - | - |
WD-L2 | - | + | - | - | - | - | - |
WD-Z1 | - | - | - | - | - | - | - |
WD-T3 | - | + | - | - | - | + | - |
WD-H1 | - | - | - | - | - | - | - |
YMA-T2 | - | + | - | - | - | - | + |
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