Identification and Control Characteristics of Antagonistic Bacteria against Tobacco Target Spot Disease

doi:10.13560/j.cnki.biotech.bull.1985.2025-0678

Abstract

Abstract:

Objective To isolate and screen biocontrol bacteria against tobacco target spot disease. Method The antagonists were isolated and screened by plate coating method and plate confrontation method from Rhododendron simsii Planch. rhizosphere. After identification by physiological and biochemical analysis and 16S rDNA gene sequence analysis, the phylogenetic tree was constructed, and the antibacterial activities of the fermented broth and the crude extract of ethyl acetate were determined concurrently. Pot culture method was used to determine the control effect of antagonists on tobacco target spot disease, as well as the effects on the activities of malondialdehyde (MDA), antioxidant enzymes and the regulation and expression of pathogenesis related protein genes. Result A strain of Burkholderia gladioli QLDJ-1 with antagonistic effect against Rhizoctonia solani was obtained. The relative inhibition rate of QLDJ-1 against R. solani was (72.107 ± 2.788) %. Compared with the control group, the disease index of target spot of tobacco plants treated with QLDJ-1 decreased by 32.02%. The activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) increased by 33.72%, 51.45% and 17.49% respectively. The content of malondialdehyde (MDA) decreased by 62.44%. The expressions of pathogenesis-related genes such as PR1b, NmIMSP, P450-1, GST and hrs203J in the tobacco plants treated with QLDJ-1 were different from those of the control. Conclusion QLDJ-1 can effectively control tobacco target spot disease and has the potential to be developed as a biocontrol agent.

Key words: rhizosphere bacteria, Burkholderia, Rhizoctonia solani, tobacco target spot disease, biological control

ZHU Zhi-yan, SONG Zhi-wei, HE Shan, ZHANG De-qing, HE Yong, TIAN Zhi-hong. Identification and Control Characteristics of Antagonistic Bacteria against Tobacco Target Spot Disease[J]. Biotechnology Bulletin, 2026, 42(4): 310-320.

Figures/Tables 11

References 40

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项目 Item	阳性/阴性 Positive （+）/ Negative （-）
生化反应 Biochemical test	+
淀粉水解 Starch hydrolysis	+
接触酶 Catalase production	+
尿素酶 Urease production	+
蔗糖利用 Sucrose utilization	-
生化反应 Biochemical test	+
脂酶 Esterase production	-
硝酸盐还原 Nitrate reduction	-
甲基红试验 Methyl red test	-
VP反应 VP test	-
柠檬酸盐利用 Citrate utilization	+

项目 Item	阳性/阴性 Positive （+）/ Negative （-）
生化反应 Biochemical test	+
淀粉水解 Starch hydrolysis	+
接触酶 Catalase production	+
尿素酶 Urease production	+
蔗糖利用 Sucrose utilization	-
生化反应 Biochemical test	+
脂酶 Esterase production	-
硝酸盐还原 Nitrate reduction	-
甲基红试验 Methyl red test	-
VP反应 VP test	-
柠檬酸盐利用 Citrate utilization	+

抗生素 Antibiotics	浓度 Concentration （μg/mL）	敏感性 Sensibility
抗生素 Antibiotics	浓度 Concentration （μg/mL）	24 h	72 h
氨苄青霉素 Ampicillin	5	+	+
	50	+	+
	100	+	+
卡那霉素 Kanamycin	5	-	+
	50	-	-
	100	-	-
潮霉素 Hygromycin	5	+	+
	50	+	+
	100	+	+
链霉素 Streptomycin	5	+	+
	50	+	+
	100	-	+

抗生素 Antibiotics	浓度 Concentration （μg/mL）	敏感性 Sensibility
抗生素 Antibiotics	浓度 Concentration （μg/mL）	24 h	72 h
氨苄青霉素 Ampicillin	5	+	+
	50	+	+
	100	+	+
卡那霉素 Kanamycin	5	-	+
	50	-	-
	100	-	-
潮霉素 Hygromycin	5	+	+
	50	+	+
	100	+	+
链霉素 Streptomycin	5	+	+
	50	+	+
	100	-	+

病原菌 Pathogenic bacteria	病害名称 Disease	最长培养时间 Maximum culture time （h）	最终抑菌圈直径 Final inhibition zone diameter （mm）	最终相对抑菌率 Final inhibition rate （%）
立枯丝核菌 Rhizoctonia solani	烟草靶斑病 Target spot disease	96	63.667±2.268aA	72.107±2.788bcB
交链格孢菌 Alternaria alternata	烟草赤星病 Brown spot	120	36.083±2.504bB	81.490±1.614abAB
茄科雷尔氏菌 Ralstonia solanacearum	烟草青枯病 Bacterial wilt	72	3.333±1.258eD	32.273±9.796eD
寄生疫霉菌 Phytophthora parasitica var. Nicotianae	烟草黑胫病 Black shank	168	31.500±1.000bcBC	79.740±0.515abcAB
立枯丝核菌 Rhizoctonia solani	水稻纹枯病 Sheath blight	72	64.438±3.710aA	71.598±4.123bcB
黄单胞菌 Xanthomonas oryzae pv	水稻白叶枯病 Bacterial leaf blight	96	5.167±1.041eD	70.637±6.870cB
炭疽病菌 Colletotrichum gloeosporioides	芒果炭疽病 Anthracnose	120	36.813±8.017bB	74.527±9.500bcAB
葡萄座腔菌 Botryosphaeria dothidea	芒果果腐病 Fruit rot	84	67.000±1.146aA	88.370±1.026aA
大斑突脐孢菌 Exserohilum turcicum	玉米大斑病 Northern leaf blight	120	29.038±1.338cdBC	78.380±0.759abcAB