CRISPR/Cas9系统在丝状真菌中的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2017-1128

摘要/Abstract

摘要： 丝状真菌（Filamentous fungi）是一种与人类生存密切相关的微生物,除了具有巨大的食用和药用价值外,还被广泛应用于工业开发。近年来,成簇的规律间隔的短回文重复序列及其相关系统（Clustered regulatory interspaced short palindromic repeats-associated protein 9,CRISPR/Cas9）以其设计简单、操作方便、精准定位、效率高等诸多优点在各个研究领域中迅速发展,并受到了国内外广泛关注,但由于某些真菌如烟曲霉中极低的同源重组率等,使得对其进行遗传操作相对困难,因而该技术在丝状真菌尤其是大型真菌中的应用鲜少。从CRISPR/Cas9技术入手,结合该技术在丝状真菌研究领域的最新应用和研究进展,对其在丝状真菌中实现编辑的各种机制做了详细介绍,并重点分析了其在各类已报道的模式真菌中的应用,旨在为更多真菌实现基因编辑提供参考。

关键词: 丝状真菌, CRISPR/Cas9, 基因编辑, 生产应用

Abstract: Filamentous fungi,which are closely related to human survival,have been widely used in industrial development and application in addition to its great edible and medicinal values. In recent years,CRISPR/Cas9 has been rapidly developed in various fields due to its simple design,convenient operation,precise positioning and high efficiency,and has been received great attentions in the world. However,because of the relatively low level of homologous recombination in some fungi,such as Aspergillus fumigatus,genetic manipulation of them is rather difficult. Therefore,this technique is rarely used in filamentous fungi,especially in large fungi. Based on the latest application and research progress of CRISPR/Cas9 technology in filamentous fungi,this paper introduced various editing mechanisms in filamentous fungi,and analyzed the reported its application in model fungi,aiming at providing a reference to conduct gene editing for more fungi.

Key words: filamentous fungi, CRISPR/Cas9, gene editing, production applications

王佩, 刘文, 沈祥陵. CRISPR/Cas9系统在丝状真菌中的研究进展[J]. 生物技术通报, 2018, 34(4): 1-8.

WANG Pei， LIU Wen， SHEN Xiang-ling. Research Progress of CRISPR/Cas9 System in Filamentous Fungi[J]. Biotechnology Bulletin, 2018, 34(4): 1-8.

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