甜瓜雄性不育系不同发育时期雄蕊转录组分析

doi:10.13560/j.cnki.biotech.bull.1985.2018-0306

生物技术通报 ›› 2018, Vol. 34 ›› Issue (8): 93-100.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0306

甜瓜雄性不育系不同发育时期雄蕊转录组分析

戴冬洋^1,3, 袁丽伟², 盛云燕³, 郑群¹

1. 石河子大学农学院,石河子 832003;
2. 河北旅游职业技术学院,承德 067000;
3. 黑龙江八一农垦大学农学院,大庆 163319

收稿日期:2018-03-30 出版日期:2018-08-26 发布日期:2018-09-04
作者简介:戴冬洋,女,硕士研究生,研究方向：蔬菜学;E-mail：1109603031@qq.com
基金资助:
国家自然科学基金项目（31401892,31772330）,黑龙江省自然科学基金项目（C2017054）,黑龙江八一农垦大学校级培育重点课题（XA201701）

Transcriptome Sequencing of Stamen in Muskmelon Male Sterile Lines at Different Developmental Stages

DAI Dong-yang^1,3, YUAN Li-wei², SHENG Yun-yan³, ZHENG Qun¹

1. College of Agronomy,Shihezi University,Shihezi 832003;
2. Hebei Tourism Vocational College,Chengde 067000;
3. College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing 163319

Received:2018-03-30 Published:2018-08-26 Online:2018-09-04

摘要/Abstract

摘要： 从分子水平探讨甜瓜雄性不育雄蕊发育不同时期差异表达基因与花粉败育的相关性,为探究甜瓜雄性不育发生机理提供依据。通过对甜瓜雄性不育系（Male sterile,MS）花蕾（直径为2和5 mm时期）雄蕊进行转录组测序,筛选差异表达基因,并对差异表达基因进行荧光定量分析。共发现224个差异表达基因,对差异表达基因进行GO功能分析显示,全部基因均归属于生物合成、分子功能和细胞组分3大分支,其中与酶类相关的差异基因为9个,2个差异表达基因为羧酸酯酶同源基因,4个为碳水化合物相关酶类的表达基因,细胞信号传导相关表达基因和玉米素生物合成相关表达基因各1个;qRT-PCR（Quantitative real-time PCR）验证表明,与碳水化合物相关酶类的表达基因和玉米素生物合成相关表达基因在雄性不育株2 mm花蕾雄蕊中的表达量均低于5 mm花蕾雄蕊中的表达量,与细胞信号传导相关表达基因在雄性不育株2 mm雄蕊中的表达量高于5 mm雄蕊中的表达量。雄性不育的发生与能量代谢相关基因表达量的上升和细胞信号传导相关表达基因表达量的减少存在一定的相关性。

关键词: 甜瓜, 雄性不育, 转录组, 差异基因

Abstract: In order to explore the mechanism of male sterility in muskmelon,the correlation between pollen abortion and differentially-expressed genes at different stages of male sterility and stamen development was studied at the molecular level. The transcriptome sequencing of bud（while its diameter was 2 mm and 5 mm）stamen from male sterile（MS）line of muskmelon was conducted,differentially-expressed genes were screened,and differentially-expressed genes were analyzed by fluorescent quantitative method. Totally 224 differentially-expressed genes were identified. GO function analysis of them showed that all the genes belonged to 3 branches of biosynthesis,molecular function and cellular components. Among them,9 differentially-expressed genes were related to various enzymes,2 related to carboxylesterase homologous,4 related to carbohydrate enzymes synthesis,and 1 gene related to signal transduction and 1 gene related to zeaxanthin biosynthesis,respectively. qRT-PCR verification revealed that the expression levels of carbohydrate-related enzymes and zeaxanthin biosynthesis-related genes in 2 mm buds and stamens were lower than those in 5 mm buds and stamens. The expressions of cell signal transduction-related genes in MS line 2 mm stamens were higher than that of 5 mm stamens. The above results indicate that there is a certain correlation between the occurrence of male sterility and the increase of energy metabolism-related gene expression and the reduction of the expression of genes related to cell signaling.

Key words: muskmelon, male sterility, transcriptome, differentially gene

戴冬洋, 袁丽伟, 盛云燕, 郑群. 甜瓜雄性不育系不同发育时期雄蕊转录组分析[J]. 生物技术通报, 2018, 34(8): 93-100.

DAI Dong-yang, YUAN Li-wei, SHENG Yun-yan, ZHENG Qun. Transcriptome Sequencing of Stamen in Muskmelon Male Sterile Lines at Different Developmental Stages[J]. Biotechnology Bulletin, 2018, 34(8): 93-100.

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甜瓜雄性不育系不同发育时期雄蕊转录组分析

Transcriptome Sequencing of Stamen in Muskmelon Male Sterile Lines at Different Developmental Stages

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