转录组与RNA-Seq 技术

生物技术通报 ›› 2012, Vol. 0 ›› Issue (12): 51-56.

转录组与RNA-Seq 技术

张春兰^{1, 2}, 秦孜娟², 王桂芝², 纪志宾², 王建民²

1 潍坊学院生物与农业工程学院，潍坊 261061 ；2 山东农业大学动物科技学院，泰安 271018

收稿日期:2012-06-04 修回日期:2013-01-25 出版日期:2012-12-26 发布日期:2013-02-06
基金资助:
: 山东省农业良种工程重大课题（2011186）

Transcriptome and RNA-Seq Technology

Zhang Chunlan^1,2, Qin Zijuan², Wang Guizhi², Ji Zhibin², Wang Jianmin²

1Biology and Agriculture Institute of Weifang University，Weifang 261061 ；2Animal Science Institute of ShandongAgricultural University，Taian 271018

Received:2012-06-04 Revised:2013-01-25 Published:2012-12-26 Online:2013-02-06

摘要/Abstract

摘要： 转录组是特定细胞或组织在特定时间或状态下转录出来的所有RNA 的集合。通过对转录组的研究可以揭示生物体的基因表达、研究结构变异及发现新基因等。转录组分析的研究方法、研究平台发生着日新月异的变化，同时生物信息学分析的内容也在逐渐完善。RNA-Seq 作为一种新的转录组研究手段，利用新一代测序技术能够更为快速、准确地为人们提供更多的生物体转录信息。主要比较近年来转录组研究的几种方法和几种RNA-Seq 的研究平台，并着重介绍RNA-Seq 的原理、用途、步骤和生物信息学分析等及在相关领域的应用等内容，为相关的研究和应用提供参考。

关键词: 转录组, RNA-Seq, 新一代测序技术

Abstract: The transcriptome is the complete set of transcripts for certain type of cells or tissues in a specific developmental stage orphysiological condition. Transcriptome analysis can reveal the organism's gene expressing level, structural variation, discovery of new genes. Theresearch methods and platforms of transcriptome are undergoing rapid changes. And bioinformatics analysis is also improved gradually. RNASeqas a new research method, it can be more quickly and afford more accurate transcriptome’s information while using the Next-generationSequencing（NGS）technology. This article compares several main methods and platforms of transcriptome in recent years, and review theprinciple, purpose, steps, bioinformatics’ analysis and applications in related fields of RNA-Seq. This will be afford useful reference for relatedresearch.

张春兰, 秦孜娟, 王桂芝, 纪志宾, 王建民. 转录组与RNA-Seq 技术[J]. 生物技术通报, 2012, 0(12): 51-56.

Zhang Chunlan, Qin Zijuan, Wang Guizhi, Ji Zhibin, Wang Jianmin. Transcriptome and RNA-Seq Technology[J]. Biotechnology Bulletin, 2012, 0(12): 51-56.

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