Progress in Fluorescence Lifetime Imaging of Circularly-permuted Fluorescent Protein Biosensors

doi:10.13560/j.cnki.biotech.bull.1985.2018-0212

Abstract

Abstract: Circularly-permuted fluorescent protein（cpFP）biosensors as novel genetically encoded biosensors consist of fluorescent protein and binding domain protein that specifically responds to typical analyte. There is relatively high sensitivity and large dynamic range during the detection of analyte duo to cpFP biosensor’ unique mode of construction,which have attracted more and more scientists’ interests,thus they have become powerful tools for monitoring life activities. Nowadays,growingly biosensors based on cpFP have been widely used in the fields of biological imaging,drug screening and cellular metabolites detection,i.e.,played an increasingly important role. However,there are issues such as development workload is very heavy,fluorescence intensity is weak,and measurement method is limited while developing these sensors. Constant efforts from scientists are still highly required to solve these issues. In this paper,we reviewed researches on the cpFP biosensors,focusing on the design method of cpFP biosensors,the types and basic properties of the current cpFP biosensors,the current states of development,and existing problems. In addition,we introduced the detection methods used in cpFP biosensors especially the fluorescence lifetime imaging method,and compared the advantages and disadvantages of these detection methods. This article aims to elaborate the development status of cpFP biosensors from various aspects,so as to provide researchers references and inspirations.

Key words: biosensor, circularly-permuted fluorescent protein, fluorescence lifetime imaging

ZHOU Jia-sheng, WANG Peng, ZHOU Huang-mei, XU Jin-ming, ZHANG San-jun. Progress in Fluorescence Lifetime Imaging of Circularly-permuted Fluorescent Protein Biosensors[J]. Biotechnology Bulletin, 2018, 34(10): 71-80.

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