Research Progress on Functional Nucleic acid Biosensors Mediated by Terminal Deoxynucleotidyl Transferase

doi:10.13560/j.cnki.biotech.bull.1985.2018-0502

Abstract

Abstract: Terminal deoxynucleotidyl transferase（TdT enzyme）is a DNA polymerase that catalyzes the binding of deoxynucleotides to the 3’ hydroxyl end of a DNA molecule,and for which a specific template is not demanded. Currently,based on the characteristics of terminal deoxynucleotidyl transferases that can extend the ends of the template nucleic acid strands and carried on with different signal outputs and amplification methods,a series of biosensing technologies were constructed,such as electrochemical biosensors,fluorescent biosensor,and surface ion resonance biosensors. This article describes the basic design principles and applications of various sensors. A series of biosensors can be designed for the detection of metal ions,pathogens,and proteins according to the properties of TdT enzymes,with the advantages of simplicity,rapidity,low cost,high sensitivity,and fine specificity. At the end of this article,the current research status on TdT-mediated biosensors is summarized and its future development direction is prospected.

Key words: terminal deoxynucleotidyl transferase, 3'-OH, deoxynucleotide, end extension, biosensor

ZHANG Yuan, LIANG Jia-hui, LUO Yun-bo, TIAN Jing-jing, TIAN Hong-tao, XU Wen-tao. Research Progress on Functional Nucleic acid Biosensors Mediated by Terminal Deoxynucleotidyl Transferase[J]. Biotechnology Bulletin, 2019, 35(1): 161-169.

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