Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (12): 29-40.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0190

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Effect of Root Rot on Arbuscular Mycorrhizal Fungi Community in Root Zone Soil of Lycium barbarum L.

LV Yan1(), WANG Wen-bin2, GOU Qi1, WANG Ying-na1, LI Jing-yu1, LIU Jian-li1()   

  1. 1. College of Biological Sciences and Engineering,North Minzu University,Yinchuan 750021
    2. College of Life Science,Hebei University,Baoding 071002
  • Received:2021-02-19 Online:2021-12-26 Published:2022-01-19
  • Contact: LIU Jian-li E-mail:lvyan0205@163.com;ljl7523@126.com

Abstract:

This paper aims to explore the effects of root rot pathogen infection on the community composition of arbuscular mycorrhizal fungi(AMF)in the root zone of Lycium barbarum L.,for laying a foundation in later applying AMF to control the root rot of L. barbarum L. The roots and soil samples of healthy and infected L. barbarum L. in three cultivation sites(Yinchuan and Zhongning county of Ningxia Hui Autonomous Region,and Jingyuan county of Gansu province)were collected. Wet sieve precipitation method and AMF colonization staining method were used to analyze the spore composition and density,mycorrhizal infection rate of AMF and the correlation between AMF diversity and soil physical and chemical factors. The results demonstrated that there was no significant difference in spore density,total mycorrhizal infection rate and T-GRSP content after the healthy L. barbarum L. plants were invaded by the pathogen of root rot,while the EE-GRSP content in Zhongning county significantly increased. The AMF community composition in the root zone soil of healthy and infected L. barbarum L. plants in different sites varied significantly(R 2=0.875,P=0.001). The dominant genus and species in Zhongning and Jingyuan county was changed,while that in Yinchuan site was unchanged. The root rot changed the soil AMF community structure in the root area of L. barbarum L. in Ningxia,such as the α-diversity increasing in Yinchuan,but no impact on the α-diversity in Zhongning and Jinghuan. There were significance among AMF spore density,species abundance,total mycorrhizal infection rate and soil physicochemical properties,and soil physicochemical properties did not affect the species diversity of AMF,but affected AMF colonization.

Key words: Lycium barbarum L., root rot, arbuscular mycorrhizal fungi, diversity