Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (2): 150-157.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0390

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Effects of Dendrobium officinale Polysaccharides on the Intestinal Mucosal Structure and Microbiota in Mice Fed a High-fat Diet

XIE Guo-zhen(), TANG Yuan, NING Xiao-mei, QIU Ji-hui, TAN Zhou-jin()   

  1. Hunan University of Chinese Medicine,Changsha 410208
  • Received:2021-03-30 Online:2022-02-26 Published:2022-03-09
  • Contact: TAN Zhou-jin E-mail:191431657@qq.com;tanzhjin@sohu.com

Abstract:

To investigate the effects of Dendrobium officinale polysaccharides(DOP)on the intestinal mucosal barrier of mice fed a high fat diet,DOP were extracted by water extraction and alcohol precipitation method. DOP were gavaged with the mice fed a high fat diet for 8 weeks,and the intestinal mucosal structure and intestinal mucosal microbiota of mice were observed. The results showed that high fat diet significantly destroyed the intestinal mucosal structure,which was characterized by intestinal mucosal atrophy,epithelial cells shedding and inflammatory exudation. Moreover,infection-associated and inflammation-related bacteria,for example,Corynebacterium_1 and Staphylococcus proliferated greatly. DOP protected intestinal mucosal structure. In addition,DOP reduced the abundance of Corynebacterium_1,increased the abundance of Candidatus_Arthromitus,and enhanced the proliferation of bacteria related to carbohydrate metabolism and short-chain fatty acid production,i.e.,Muribaculaceae,Bacteroides and Lachnospiraceae_NK4A136_group. The results showed that DOP could protect the intestinal mucosal barrier by maintaining the structural integrity of intestinal mucosa,regulating the composition of intestinal mucosal microbiota,promoting carbohydrate metabolism,and producing short-chain fatty acids.

Key words: Dendrobium officinale polysaccharides, high fat diet, intestinal mucosa, microbiota, diversity