Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (7): 150-162.doi: 10.13560/j.cnki.biotech.bull.1985.2023-1090
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ZHANG Ming-ya(), PANG Sheng-qun(), LIU Yu-dong, SU Yong-feng, NIU Bo-wen, HAN Qiong-qiong
Received:
2023-11-20
Online:
2024-07-26
Published:
2024-07-30
Contact:
PANG Sheng-qun
E-mail:2419538996@qq.com;pangshqok@shzu.edu.cn
ZHANG Ming-ya, PANG Sheng-qun, LIU Yu-dong, SU Yong-feng, NIU Bo-wen, HAN Qiong-qiong. Identification and Expression Analysis of FAD Gene Family in Solanum lycopersicum[J]. Biotechnology Bulletin, 2024, 40(7): 150-162.
基因名称Gene name | 上游引物Forward primer(5'-3') | 下游引物Reverse primer(5'-3') |
---|---|---|
Actin | ATCTTGGCTTCCCTCAGCAC | GCATCTCTGGTCCAGTAGGAAAT |
SlFAD1 | TCTACGGTGTGCCCCTTCTA | AGTGATGGGTGAGTGTGCTG |
SlFAD4 | TCAAGAAAGCCATCCCACCC | TGGCAGATCCAGTAAAGCGG |
SlFAD6 | CTCTTATCACCACCGCCCTC | GCTGCATTCACGATTGGCAT |
SlFAD14 | CTTCCGTTTCTGTTTCCGCC | TAAGCGGTGCACTTACCCTC |
SlFAD18 | CACCTGGTCTGTGGGAGTTC | CCAGCCCCAGAAGTAAACCA |
SlFAD21 | CGAGGTTGAGGTGCTTGACT | TTCCAGGTTGAGTCGGATGC |
SlFAD23 | CCGTTACTTTTGCATGGCCT | CCCAAGCTAGCCCTTTCATC |
Table 1 Primers for RT-qPCR
基因名称Gene name | 上游引物Forward primer(5'-3') | 下游引物Reverse primer(5'-3') |
---|---|---|
Actin | ATCTTGGCTTCCCTCAGCAC | GCATCTCTGGTCCAGTAGGAAAT |
SlFAD1 | TCTACGGTGTGCCCCTTCTA | AGTGATGGGTGAGTGTGCTG |
SlFAD4 | TCAAGAAAGCCATCCCACCC | TGGCAGATCCAGTAAAGCGG |
SlFAD6 | CTCTTATCACCACCGCCCTC | GCTGCATTCACGATTGGCAT |
SlFAD14 | CTTCCGTTTCTGTTTCCGCC | TAAGCGGTGCACTTACCCTC |
SlFAD18 | CACCTGGTCTGTGGGAGTTC | CCAGCCCCAGAAGTAAACCA |
SlFAD21 | CGAGGTTGAGGTGCTTGACT | TTCCAGGTTGAGTCGGATGC |
SlFAD23 | CCGTTACTTTTGCATGGCCT | CCCAAGCTAGCCCTTTCATC |
保守基序名称 Conservative motif name | 具体序列分布情况 Specific sequence distribution |
---|---|
Motif 1 | WVTAHECGHHAFSDYQWJBDTVGFILHSSJLVPYFSWKYSHRRHHSNTGS |
Motif 2 | NKVFHNITDTHVLHHLFSTIPHYHAVEATKAIKPLLGEYYQFDGTPIYKA |
Motif 3 | EVKKHNKAKDCWJIISGKVYDVTKFLDDHPGGDEVLLSATG |
Motif 4 | RVPSSKPPFTJSDIKKAIPPHCFQRSLVRSFSYVVRDJVLVFILYYIAAT |
Motif 5 | CIYGVPLLIVNGFIVLITYLHHTHASLPHYDSSEWDWLRGA |
Motif 6 | NVSGRPYDRFASHYBPYSPJYNDRERLQI |
Motif 7 | KDATDDFEDVGHSSSAREMLDKYYIGEIDSSTIPTKRKYTP |
Motif 8 | IWRDFKECIYVEKDEESQDKGVFWYKNK |
Motif 9 | PYNYLAWPIYWIAQGCVFTGI |
Motif 10 | QPKGNDWFEKQTAGTIDIACSPQMDWFFGGLQFQLEHHLFPRLPRCQLRK |
Table 2 Distribution of conservative motif sequence
保守基序名称 Conservative motif name | 具体序列分布情况 Specific sequence distribution |
---|---|
Motif 1 | WVTAHECGHHAFSDYQWJBDTVGFILHSSJLVPYFSWKYSHRRHHSNTGS |
Motif 2 | NKVFHNITDTHVLHHLFSTIPHYHAVEATKAIKPLLGEYYQFDGTPIYKA |
Motif 3 | EVKKHNKAKDCWJIISGKVYDVTKFLDDHPGGDEVLLSATG |
Motif 4 | RVPSSKPPFTJSDIKKAIPPHCFQRSLVRSFSYVVRDJVLVFILYYIAAT |
Motif 5 | CIYGVPLLIVNGFIVLITYLHHTHASLPHYDSSEWDWLRGA |
Motif 6 | NVSGRPYDRFASHYBPYSPJYNDRERLQI |
Motif 7 | KDATDDFEDVGHSSSAREMLDKYYIGEIDSSTIPTKRKYTP |
Motif 8 | IWRDFKECIYVEKDEESQDKGVFWYKNK |
Motif 9 | PYNYLAWPIYWIAQGCVFTGI |
Motif 10 | QPKGNDWFEKQTAGTIDIACSPQMDWFFGGLQFQLEHHLFPRLPRCQLRK |
基因名称Gene name | 脱落酸响应Abscisic acid response | 厌氧诱导响应Anaerobic induction | 生长素响应Auxin response | 昼夜节律调控Circadian control | 防御和应激响应 Defense and stress response | 干旱诱导响应Drought- inducibility | 低温响应Low-temperature response | 赤霉素响应Gibberellin- response | 分生组织表达Meristem expression | 水杨酸响应Salicylic acid response | 茉莉酸响应MeJA- response |
---|---|---|---|---|---|---|---|---|---|---|---|
SlFAD1 | 5 | 4 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 2 | 8 |
SlFAD2 | 0 | 5 | 1 | 0 | 1 | 2 | 1 | 0 | 1 | 0 | 6 |
SlFAD3 | 4 | 1 | 0 | 1 | 1 | 1 | 4 | 2 | 0 | 1 | 8 |
SlFAD4 | 8 | 3 | 2 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 6 |
SlFAD5 | 13 | 3 | 2 | 0 | 1 | 2 | 0 | 2 | 1 | 1 | 6 |
SlFAD6 | 1 | 3 | 2 | 1 | 1 | 4 | 0 | 2 | 1 | 2 | 8 |
SlFAD7 | 9 | 5 | 2 | 0 | 1 | 2 | 1 | 3 | 2 | 2 | 10 |
SlFAD8 | 8 | 4 | 2 | 0 | 0 | 2 | 3 | 2 | 0 | 2 | 6 |
SlFAD9 | 4 | 2 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 2 | 6 |
SlFAD10 | 4 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 4 |
SlFAD11 | 3 | 3 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 4 |
SlFAD12 | 3 | 1 | 0 | 1 | 2 | 0 | 2 | 1 | 1 | 3 | 6 |
SlFAD13 | 8 | 2 | 1 | 1 | 0 | 1 | 3 | 3 | 1 | 0 | 0 |
SlFAD14 | 5 | 3 | 1 | 0 | 2 | 5 | 2 | 4 | 1 | 0 | 4 |
SlFAD15 | 4 | 2 | 3 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 8 |
SlFAD16 | 11 | 2 | 2 | 0 | 0 | 0 | 1 | 2 | 1 | 4 | 16 |
SlFAD17 | 3 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 |
SlFAD18 | 1 | 6 | 1 | 1 | 1 | 2 | 2 | 2 | 1 | 1 | 6 |
SlFAD19 | 14 | 1 | 5 | 1 | 1 | 1 | 0 | 1 | 4 | 0 | 24 |
SlFAD20 | 10 | 7 | 1 | 1 | 2 | 0 | 3 | 2 | 0 | 0 | 2 |
SlFAD21 | 5 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 6 |
SlFAD22 | 5 | 1 | 0 | 3 | 1 | 0 | 1 | 3 | 2 | 2 | 6 |
SlFAD23 | 4 | 3 | 1 | 1 | 0 | 0 | 2 | 2 | 0 | 2 | 10 |
SlFAD24 | 3 | 6 | 0 | 3 | 2 | 2 | 3 | 0 | 0 | 2 | 8 |
SlFAD25 | 1 | 4 | 2 | 1 | 2 | 1 | 0 | 2 | 2 | 1 | 4 |
SlFAD26 | 0 | 6 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 8 |
Table 3 Analysis of promoter cis-acting elements of the tomato SlFAD gene family
基因名称Gene name | 脱落酸响应Abscisic acid response | 厌氧诱导响应Anaerobic induction | 生长素响应Auxin response | 昼夜节律调控Circadian control | 防御和应激响应 Defense and stress response | 干旱诱导响应Drought- inducibility | 低温响应Low-temperature response | 赤霉素响应Gibberellin- response | 分生组织表达Meristem expression | 水杨酸响应Salicylic acid response | 茉莉酸响应MeJA- response |
---|---|---|---|---|---|---|---|---|---|---|---|
SlFAD1 | 5 | 4 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 2 | 8 |
SlFAD2 | 0 | 5 | 1 | 0 | 1 | 2 | 1 | 0 | 1 | 0 | 6 |
SlFAD3 | 4 | 1 | 0 | 1 | 1 | 1 | 4 | 2 | 0 | 1 | 8 |
SlFAD4 | 8 | 3 | 2 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 6 |
SlFAD5 | 13 | 3 | 2 | 0 | 1 | 2 | 0 | 2 | 1 | 1 | 6 |
SlFAD6 | 1 | 3 | 2 | 1 | 1 | 4 | 0 | 2 | 1 | 2 | 8 |
SlFAD7 | 9 | 5 | 2 | 0 | 1 | 2 | 1 | 3 | 2 | 2 | 10 |
SlFAD8 | 8 | 4 | 2 | 0 | 0 | 2 | 3 | 2 | 0 | 2 | 6 |
SlFAD9 | 4 | 2 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 2 | 6 |
SlFAD10 | 4 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 4 |
SlFAD11 | 3 | 3 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 4 |
SlFAD12 | 3 | 1 | 0 | 1 | 2 | 0 | 2 | 1 | 1 | 3 | 6 |
SlFAD13 | 8 | 2 | 1 | 1 | 0 | 1 | 3 | 3 | 1 | 0 | 0 |
SlFAD14 | 5 | 3 | 1 | 0 | 2 | 5 | 2 | 4 | 1 | 0 | 4 |
SlFAD15 | 4 | 2 | 3 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 8 |
SlFAD16 | 11 | 2 | 2 | 0 | 0 | 0 | 1 | 2 | 1 | 4 | 16 |
SlFAD17 | 3 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 |
SlFAD18 | 1 | 6 | 1 | 1 | 1 | 2 | 2 | 2 | 1 | 1 | 6 |
SlFAD19 | 14 | 1 | 5 | 1 | 1 | 1 | 0 | 1 | 4 | 0 | 24 |
SlFAD20 | 10 | 7 | 1 | 1 | 2 | 0 | 3 | 2 | 0 | 0 | 2 |
SlFAD21 | 5 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 6 |
SlFAD22 | 5 | 1 | 0 | 3 | 1 | 0 | 1 | 3 | 2 | 2 | 6 |
SlFAD23 | 4 | 3 | 1 | 1 | 0 | 0 | 2 | 2 | 0 | 2 | 10 |
SlFAD24 | 3 | 6 | 0 | 3 | 2 | 2 | 3 | 0 | 0 | 2 | 8 |
SlFAD25 | 1 | 4 | 2 | 1 | 2 | 1 | 0 | 2 | 2 | 1 | 4 |
SlFAD26 | 0 | 6 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 8 |
Fig. 6 Analysis of expression patterns of tomato SlFAD gene family members in different tissues and at 10℃ Different letters indicate significant differences between treatments at the 0.05 level
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