Genetic Diversity Analysis of Stropharia rugosoannulata Germplasm Resources Based on ISSR Markers and Agronomic Traits

doi:10.13560/j.cnki.biotech.bull.1985.2025-1351

Abstract

Abstract:

Objective Commercial cultivated populations of Stropharia rugosoannulata face challenges including genetic admixture and a lack of superior varieties. This study aimed to characterize the genetic diversity and phenotypic variation of cultivated strains from diverse geographic origins, screen for elite germplasm, and provide a scientific foundation for variety improvement. Method 67 S. rugosoannulata strains were collected from different geographical regions. Genetic diversity was analyzed using 10 ISSR primers, and 10 agronomic traits were measured. A comprehensive evaluation was conducted by combining correlation analysis, principal component analysis, and cluster analysis. Result A total of 65 clear bands were amplified by ISSR markers, among which 51 were polymorphic, with the percentage of polymorphic loci per primer ranging from 55.56% to 100%. The average Nei’s gene diversity index and Shannon diversity index were 0.27 and 0.43, respectively. UPGMA cluster analysis identified five clusters, with genetic similarity coefficients ranging from 0.43 to 0.96. Principal component analysis accounted for 28% of the total genetic variation, and strains within the core clusters were closely clustered and shared highly similar genetic backgrounds. For agronomic traits, pileus hardness exhibited the highest coefficient of variation (124.03%), while yield had the highest Shannon diversity index (1.88). Yield was extremely significantly positively correlated with seven traits, including single fruiting body weight and pileus diameter (P<0.01), whereas the number of hyphal clamp connections showed no significant correlation with any agronomic trait. The first three phenotypic principal components contributed 74.51% of the total cumulative variance, and pileus diameter and stipe diameter were identified as the core indicators for phenotypic evaluation. Three strains (BIPP21340027, BIPP21340016, and BIPP21340020) ranked top in the comprehensive evaluation, featuring both high yield and excellent commercial traits. Conclusion This study revealed that the tested Stropharia rugosoannulata strains have a narrow genetic base yet rich phenotypic variation. Pileus diameter and stipe diameter were designated as the core indicators for phenotypic evaluation, and three elite strains were screened out. The results provide a reference for screening superior germplasm, selecting hybrid parents, and breeding new varieties of Stropharia rugosoannulata.

Key words: Stropharia rugosoannulata, ISSR, agronomic traits, genetic diversity, comprehensive evaluation

LI Shi-qi, WU Yue-yuan, ZHANG Dian-peng, GUO Yuan, LIU Yu, SONG Zhong-juan, WANG Shou-xian, SONG Shuang. Genetic Diversity Analysis of Stropharia rugosoannulata Germplasm Resources Based on ISSR Markers and Agronomic Traits[J]. Biotechnology Bulletin, doi: 10.13560/j.cnki.biotech.bull.1985.2025-1351.

Figures/Tables 11

References 35

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菌株编号Strain number	来源Origin	菌株编号Strain number	来源Origin	菌株编号Strain number	来源Origin	菌株编号Strain number	来源Origin
BIPP21340001	北京市海淀区	BIPP21340023	北京市密云区	BIPP21340040	北京市房山区	BIPP21340061	山东省
BIPP21340002	北京市通州区	BIPP21340024	北京市密云区	BIPP21340041	河北邢台临县	BIPP21340062	山东省
BIPP21340004	北京市房山区	BIPP21340025	北京市房山区	BIPP21340042	河北邢台临县	BIPP21340063	山东省
BIPP21340005	北京市房山区	BIPP21340026	北京市房山区	BIPP21340043	北京市房山区	BIPP21340064	山东省
BIPP21340008	北京市密云区	BIPP21340027	北京市密云区	BIPP21340044	陕西省安康市	BIPP21340065	山东省
BIPP21340010	北京市大兴区	BIPP21340028	北京市房山区	BIPP21340045	福建省	BIPP21340066	新疆维吾尔自治区
BIPP21340011	北京市大兴区	BIPP21340029	北京市顺义区	BIPP21340050	福建省	BIPP21340067	新疆维吾尔自治区
BIPP21340012	北京市房山区	BIPP21340030	北京市平谷区	BIPP21340051	山东省聊城市	BIPP21340068	新疆维吾尔自治区
BIPP21340013	北京市丰台区	BIPP21340031	北京市平谷区	BIPP21340052	北京市海淀区	BIPP21340069	山东省
BIPP21340015	北京市顺义区	BIPP21340032	北京市丰台区	BIPP21340053	四川省	BIPP21340070	北京市海淀区
BIPP21340016	北京市顺义区	BIPP21340033	北京市大兴区	BIPP21340054	四川省	BIPP21340072	北京市海淀区
BIPP21340017	北京市顺义区	BIPP21340034	北京市海淀区	BIPP21340055	山东省	BIPP21340074	福建省
BIPP21340018	北京市顺义区	BIPP21340035	北京市海淀区	BIPP21340056	山东省	BIPP21340081	云南省
BIPP21340019	北京市顺义区	BIPP21340036	北京市房山区	BIPP21340057	黑龙江省	BIPP21340082	云南省
BIPP21340020	北京市顺义区	BIPP21340037	北京市房山区	BIPP21340058	黑龙江省	BIPP21340085	山东省
BIPP21340021	北京市平谷区	BIPP21340038	北京市房山区	BIPP21340059	山东省	BIPP21340090	黑龙江省
BIPP21340022	北京市延庆区	BIPP21340039	北京市海淀区	BIPP21340060	山东省

菌株编号Strain number	来源Origin	菌株编号Strain number	来源Origin	菌株编号Strain number	来源Origin	菌株编号Strain number	来源Origin
BIPP21340001	北京市海淀区	BIPP21340023	北京市密云区	BIPP21340040	北京市房山区	BIPP21340061	山东省
BIPP21340002	北京市通州区	BIPP21340024	北京市密云区	BIPP21340041	河北邢台临县	BIPP21340062	山东省
BIPP21340004	北京市房山区	BIPP21340025	北京市房山区	BIPP21340042	河北邢台临县	BIPP21340063	山东省
BIPP21340005	北京市房山区	BIPP21340026	北京市房山区	BIPP21340043	北京市房山区	BIPP21340064	山东省
BIPP21340008	北京市密云区	BIPP21340027	北京市密云区	BIPP21340044	陕西省安康市	BIPP21340065	山东省
BIPP21340010	北京市大兴区	BIPP21340028	北京市房山区	BIPP21340045	福建省	BIPP21340066	新疆维吾尔自治区
BIPP21340011	北京市大兴区	BIPP21340029	北京市顺义区	BIPP21340050	福建省	BIPP21340067	新疆维吾尔自治区
BIPP21340012	北京市房山区	BIPP21340030	北京市平谷区	BIPP21340051	山东省聊城市	BIPP21340068	新疆维吾尔自治区
BIPP21340013	北京市丰台区	BIPP21340031	北京市平谷区	BIPP21340052	北京市海淀区	BIPP21340069	山东省
BIPP21340015	北京市顺义区	BIPP21340032	北京市丰台区	BIPP21340053	四川省	BIPP21340070	北京市海淀区
BIPP21340016	北京市顺义区	BIPP21340033	北京市大兴区	BIPP21340054	四川省	BIPP21340072	北京市海淀区
BIPP21340017	北京市顺义区	BIPP21340034	北京市海淀区	BIPP21340055	山东省	BIPP21340074	福建省
BIPP21340018	北京市顺义区	BIPP21340035	北京市海淀区	BIPP21340056	山东省	BIPP21340081	云南省
BIPP21340019	北京市顺义区	BIPP21340036	北京市房山区	BIPP21340057	黑龙江省	BIPP21340082	云南省
BIPP21340020	北京市顺义区	BIPP21340037	北京市房山区	BIPP21340058	黑龙江省	BIPP21340085	山东省
BIPP21340021	北京市平谷区	BIPP21340038	北京市房山区	BIPP21340059	山东省	BIPP21340090	黑龙江省
BIPP21340022	北京市延庆区	BIPP21340039	北京市海淀区	BIPP21340060	山东省

引物名称 Primer name	引物序列 Primer sequence （5′-3′）
ISSR1	CTCCTCCTCCTCSC
ISSR2	BDBACAACAACAACAACA
ISSR4	CACCACCACCACSC
ISSR5	VDHTCGTCGTCGTCGTCG
ISSR6	DHBCGACGACGACGACGA
ISSR12	AGAGAGAGAGAGAGAGT
ISSR13	GAGAGAGAGAGAGAGAC
ISSR15	CACACACACACACACAG
ISSR17	TGTGTGTGTGTGTGTGRC
ISSR18	VHVGTGTGTGTGTGTGTGT

引物名称 Primer name	引物序列 Primer sequence （5′-3′）
ISSR1	CTCCTCCTCCTCSC
ISSR2	BDBACAACAACAACAACA
ISSR4	CACCACCACCACSC
ISSR5	VDHTCGTCGTCGTCGTCG
ISSR6	DHBCGACGACGACGACGA
ISSR12	AGAGAGAGAGAGAGAGT
ISSR13	GAGAGAGAGAGAGAGAC
ISSR15	CACACACACACACACAG
ISSR17	TGTGTGTGTGTGTGTGRC
ISSR18	VHVGTGTGTGTGTGTGTGT

引物Primer	扩增出的条带数 Number of amplified bands	多态性条带数 Number of polymorphic bands	多态位点百分率Percentage of polymorphic loci	观测等位基因数 Observed number of alleles	有效等位基因数 Effective number of alleles	Nei’s基因多样性指数 Nei’s gene diversity index	香农多样性指数 Shannon diversity index（I）
ISSR1	9	8	88.89%	1.89	1.59	0.34	0.67
ISSR2	7	6	85.71%	1.86	1.51	0.31	0.47
ISSR4	6	4	66.67%	1.67	1.29	0.18	0.29
ISSR5	7	5	71.43%	1.71	1.43	0.27	0.40
ISSR6	9	5	55.56%	1.56	1.22	0.15	0.23
ISSR12	5	3	60.00%	1.60	1.33	0.21	0.32
ISSR13	7	7	100%	2.00	1.83	0.45	0.64
ISSR15	4	4	100%	2.00	1.59	0.34	0.51
ISSR17	6	6	100%	2.00	1.59	0.36	0.55
ISSR18	5	3	60.00%	1.60	1.16	0.12	0.19