Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (3): 65-69.

• Research report • Previous Articles     Next Articles

Analysis of Seven Big Spike Types from the Hybrids of Wheat-Rye Substitution Lines by Morphology and SSR

Jiang Luming, Tian Chao, Li Jilin, Zhang Yanming   

  1. Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province,College of Life Science and Technology, Harbin Normal University, Harbin 150025）
  • Received:2012-11-26 Revised:2013-03-21 Online:2013-03-20 Published:2013-03-21

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Abstract: We analyzed the big spike lines 1-5, 1-6, 1-7, 1-8, 1-9, 1-10 and 1-11 by morphology and SSR, which were bred from hybrids of wheat-rye substitution lines 5R/5A and 1R/1D. The results showed that the genetic performance of seven strains were stability, and had the good rye traits, such as big spike, resistance to disease. Seven lines contained genetic element of rye chromosome that were determined by ryespecific primers PSC119.1. The amplification results of primer SCM138 showed that 1-6, 1-7, 1-8, 1-9 and 1-10 contained chromatin of 5RS. The primer SCM120 and TSM604 could amplify rye specific fragment stably in seven lines that proved the lines contained the rye chromatin of 5RL and 1RS. These results provide the material foundation for wheat genetic breeding and quality improvement.

Key words: Wheat, Rye, Substitution lines, SSR