Cloning and Tissue Expression Analysis of CSRP3 in Yak

doi:10.13560/j.cnki.biotech.bull.1985.2019-0873

Abstract

Abstract: The CSRP3 gene（cysteine and glycine-rich protein 3）encodes CRP3 protein,which is a positive regulator of myogenesis and plays an important role in muscle development and structural maintenance of muscle cells. Through cloning and tissue expression profile analysis of yak CSRP3 gene,the basic data may be provided for the further study of improving yak meat quality. The CDS region of yak CSRP3 gene was cloned by RT-PCR and analyzed by bioinformatics of sequence analysis,protein structure and function prediction. Finally,the expression of the gene in yak tissues was detected by qPCR. The results showed that the CDS region length of CSRP3 gene in yak was 585 bp,and a total of 194 amino acids were encoded. The phylogenetic tree demonstrated that yak had the closest relationship with cattle,followed by sheep. The protein encoded by CSRP3 gene was an alkaline unstable hydrophilic protein without transmembrane structure and signal peptide,not a secretory protein. There were 22 phosphorylation sites,2 N-glycosylation sites,7 O-glycosylation sites and 2 LIM domains,and it belonged to the superfamily member of LIM structure domain protein. The protein was mainly distributed in the nucleus. The secondary structure was mainly composed of random coils. The best model of the tertiary structure was 1b8t.1.A. The bioinformatics analysis revealed that the CRP3 protein contained 2 LIM domains and mainly distributed in the nucleus. The qPCR results showed that the yak CSRP3 gene had higher expression in the gluteus maximus. These results provide basic data for the research on whether the CSRP3 gene may improve the quality of yak meat.

Key words: Yak, CSRP3 gene, tissue expression, bioinformatics analysis

BAI Jia-ling, WANG Hui, CHAI Zhi-xin, WANG Ji-kun, WANG Jia-bo, WU Zhi-juan, XIN Jin-wei, ZHONG Jin-cheng, CHEN Zhi-hua, JI Qiu-mei. Cloning and Tissue Expression Analysis of CSRP3 in Yak[J]. Biotechnology Bulletin, 2020, 36(6): 200-207.

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