不同产地油茶籽油提取物的抗氧化活性比较分析及其营养评价

doi:10.13560/j.cnki.biotech.bull.1985.2019-0433

摘要/Abstract

摘要： 为探究海南油茶籽油（茶油）与大陆优良品系茶油间品质差异,采用气相色谱-质谱联用（GC-MS）、高效液相色谱技术（HPLC）对其脂肪酸和活性成分进行检测,并通过4种分析方法评估茶油提取物的抗氧化能力。结果表明,海南（炒）茶油油酸含量（81.353%）显著高于江西（蒸）茶油（70.849%）,亚油酸在海南（蒸）茶油中含量最高（8.080%）,而该成分在海南（炒）茶油中却未检测出。此外,在样品中还鉴定到角鲨烯（2.300%-15.237%）、维生素E（0.400%-2.900%）、豆甾醇（0%-2.507%）及茶多酚（6种）等次生代谢产物,其中,海南（炒）茶油提取物中槲皮素含量高达59.025 µg/g,约为海南（蒸）茶油和江西（蒸）茶油的20倍,差异显著。供试茶油均具有一定程度的抗氧化活性,呈剂量依赖关系,相比其他3种茶油,海南（炒）茶油具有更强的自由基清除能力和还原能力,说明此栽培种具有潜在的开发利用价值和医用经济效益。

关键词: 油茶籽油, 气相色谱-质谱联用, 高效液相色谱, 脂肪酸, 活性成分, 抗氧化能力

Abstract: In order to investigate the quality differences of Camellia oleifera oils between from Hainan Island and other areas,gas chromatography mass spectrometry（GC-MS）and high performance liquid chromatography（HPLC）were used to determine their fatty acids and active ingredients,and their antioxidant activities were evaluated by applying 4 analytical methods. The results indicated that the content of oleic acid（81.353%）in Hainan（stir-fried）oil was significantly higher than that in Jiangxi（steamed）oil（70.849%）,and linoleic acid content（8.080%）was the highest in Hainan（steamed）oil,while it was not detected in Hainan（stir-fried）oil. In addition,some secondary metabolites such as squalene（2.300%-15.237%）,vitamin E（0.400%-2.900%）,stigmasterol（0%-2.507%）and 6 kinds of polyphenols were detected in the samples,and the level of quercetin in Hainan（stir-fried）oil extract was 59.025 µg/g,which was about 20 times higher than those in Hainan（steamed）oil and Jiangxi（steamed）oil,thus the difference was significant. Furthermore,the oil extracts of C. oleifera exhibited clear antioxidant activities in a dose-dependent manner,among them,the free radical scavenging capacity of Hainan（stir-fried）oil exceeded those of the other samples,concluding that this cultivar had potential utilization values as well as medical and economic benefits.

Key words: Camellia oleifera oil, GC-MS, HPLC, fatty acids, active ingredients, antioxidant activities

叶洲辰, 吴友根, 于靖, 张军锋, 杨东梅, 胡新文. 不同产地油茶籽油提取物的抗氧化活性比较分析及其营养评价[J]. 生物技术通报, 2019, 35(10): 80-88.

YE Zhou-chen, WU You-gen, YU Jing, ZHANG Jun-feng, YANG Dong-mei, HU Xin-wen. Comparative Analysis of Antioxidant Activities of Camellia oleifera Oil Extracts from Different Areas and Their Nutritional Assessments[J]. Biotechnology Bulletin, 2019, 35(10): 80-88.

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